Frayne Consultants

Phosphate analogues that block nuclease acitivity in and out of cells. 
mRNA accumulator for the rapid increase in mRNA levels in bacteria
TLR9 agonist via modified single-stranded phage DNA



VivoSyn® Synthetic Biology Tools


Our phosphate analogues, are for use in all cells and cell systems, and enable you to create chemically modified nucleic acids in vivo with nuclease resistant backbones that have metabolic consequences in cells leading to enhanced biosynthesis, protein secretion, and/or differentiation.     

mRNA Accumulator: rapidly increase mRNA in bacteria with only a 1 hr. incubation. Some mRNAs reach rRNA levels and can serve as markers for species, strain, or pathogen detection such as for E. coli. Many high level mRNAs correspond to mobile genetic elements making them great for monitoring strains and pathogens as well. Overall, mRNAs increased on average ~10X fold.  Also protects during RNA isolation improving your results.

Differentiation & Protein Synthesis: in general compound promotes enhanced differentiation and/or bio-synthetic processes. Use results in transcriptional changes resulting in a dramatic global shift in pattern of protein expression, showing greater increases for proteins of lower abundance, although abundant proteins can also be significantly enhanced.

Cell Secretion: use enhances total protein secretion and/or metabolic secretion depending on the organism. Yeast show enhanced protein secretion of 2.4X fold while Bacillus show enhanced metabolic secretion.

Metabolic Engineering: RNA stabilization results in energy savings that are used by cells to increase bio-synthetic processes such as fatty acid synthesis, isoprenoid synthesis, and carbohydrate synthesis in E. coli. Also results in an increase in PRPP derivatives due to decreased RNA turnover in various bacteria.

Chemically Modified DNA: microbial production of thio-modified DNA, especially single-stranded phage DNA that can be modified with fluorescent tags, etc. for use in DNA probes. RNA is also modified and stable. ssDNA can also be used as a TLR9 agonist for vaccine adjuvants or as an immunomodulatory agent.

Mutagenesis: non-toxic approach for enhanced mutagenesis in bacteria only, not in eukaryotes